Journal: BioMed Research International

Article Title: Fibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial Cells

doi: 10.1155/2013/431465

Figure Lengend Snippet: Biofilm formation by S. mutans following growth in bovine plasma as quantified by crystal violet staining. Results are expressed as means ± standard deviations of triplicate assays from two independent experiments. * P < 0.01 and ** P < 0.001: significantly different from the control without bovine plasma (growth in THB-HK medium).

Article Snippet: Eight strains of S. mutans (ATCC 25175, ATCC 31383, ATCC 35668, IFN, NY257, UA96, 12A, and 33A) were used in this study.

Techniques: Clinical Proteomics, Staining, Control

Journal: BioMed Research International

Article Title: Fibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial Cells

doi: 10.1155/2013/431465

Figure Lengend Snippet: Effect of various plasma proteins added to THB-HK medium on biofilm formation by S. mutans . Results are expressed as means ± standard deviations of triplicate assays from two independent experiments. * P < 0.001: significantly different from the control (without plasma proteins).

Article Snippet: Eight strains of S. mutans (ATCC 25175, ATCC 31383, ATCC 35668, IFN, NY257, UA96, 12A, and 33A) were used in this study.

Techniques: Clinical Proteomics, Control

Journal: BioMed Research International

Article Title: Fibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial Cells

doi: 10.1155/2013/431465

Figure Lengend Snippet: Effect of various concentrations of human fibrinogen added to THB-HK medium on biofilm formation by S. mutans . Results are expressed as means ± standard deviations of triplicate assays from two independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001: significantly different from the control (without fibrinogen).

Article Snippet: Eight strains of S. mutans (ATCC 25175, ATCC 31383, ATCC 35668, IFN, NY257, UA96, 12A, and 33A) were used in this study.

Techniques: Control

Journal: BioMed Research International

Article Title: Fibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial Cells

doi: 10.1155/2013/431465

Figure Lengend Snippet: Effect of human fibrinogen (5 mg mL −1 ) added to THB-HK medium on biofilm formation by various strains of S. mutans . Results are expressed as means ± standard deviations of triplicate assays. * P < 0.01: significantly different from the control (without fibrinogen).

Article Snippet: Eight strains of S. mutans (ATCC 25175, ATCC 31383, ATCC 35668, IFN, NY257, UA96, 12A, and 33A) were used in this study.

Techniques: Control

Journal: BioMed Research International

Article Title: Fibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial Cells

doi: 10.1155/2013/431465

Figure Lengend Snippet: Scanning electron micrographs of S. mutans biofilms formed on plastic tissue culture coverslips following growth in THB-HK medium (a), 50% bovine plasma (b), THB-HK medium + fibrinogen (0.156 mg mL −1 ) (c), and THB-HK medium + sucrose (0.25%) (d). Magnification 1000x.

Article Snippet: Eight strains of S. mutans (ATCC 25175, ATCC 31383, ATCC 35668, IFN, NY257, UA96, 12A, and 33A) were used in this study.

Techniques: Clinical Proteomics

Journal: BioMed Research International

Article Title: Fibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial Cells

doi: 10.1155/2013/431465

Figure Lengend Snippet: Effect of various concentrations of sucrose added to THB-HK medium in the presence and absence of fibrinogen on biofilm formation by S. mutans . Results are expressed as means ± standard deviations of triplicate assays from two independent experiments. * P < 0.01 and ** P < 0.001: significantly different from the control (without fibrinogen).

Article Snippet: Eight strains of S. mutans (ATCC 25175, ATCC 31383, ATCC 35668, IFN, NY257, UA96, 12A, and 33A) were used in this study.

Techniques: Control

Journal: BioMed Research International

Article Title: Fibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial Cells

doi: 10.1155/2013/431465

Figure Lengend Snippet: Effects of fibrinogen on the adherence of S. mutans to human endothelial cells. Results are expressed as means ± standard deviations of triplicate assays from two independent experiments. A value of 100% was assigned to the control (without fibrinogen). * P < 0.05 and ** P < 0.01: significantly different from the control (without fibrinogen).

Article Snippet: Eight strains of S. mutans (ATCC 25175, ATCC 31383, ATCC 35668, IFN, NY257, UA96, 12A, and 33A) were used in this study.

Techniques: Control

Journal: BioMed Research International

Article Title: Fibrinogen-Induced Streptococcus mutans Biofilm Formation and Adherence to Endothelial Cells

doi: 10.1155/2013/431465

Figure Lengend Snippet: Effect of fibrinogen on the binding of fibrinogen-Alexa Fluor conjugate to S. mutans cells. * P < 0.01: significantly different from the control (without nonfluorescent fibrinogen).

Article Snippet: Eight strains of S. mutans (ATCC 25175, ATCC 31383, ATCC 35668, IFN, NY257, UA96, 12A, and 33A) were used in this study.

Techniques: Binding Assay, Control

Journal: Journal of Dental Sciences

Article Title: Effect of brushing on surface roughness, fluoride release, and biofilm formation with different tooth-colored materials

doi: 10.1016/j.jds.2021.08.013

Figure Lengend Snippet: Biovolume (μm 3 μm −2 ) (means ± standard deviation) of S. mutans biofilms on the tested materials.

Article Snippet: The cariogenic bacteria S. mutans (ATCC 25175, Thai Can Biotech, Bangkok, Thailand) were cultured in brain-heart infusion agar (BHI) in a 5% CO 2 incubator at 37 °C for 48 h. Bacteria were then cultivated in brain-heart infusion broth supplemented with 5% sucrose in 5% CO 2 at 37 °C to achieve the desired turbidity at a cell density of 1 × 10 8 CFU/ml or 0.5 McFarland.

Techniques: Standard Deviation

Journal: Journal of Dental Sciences

Article Title: Effect of brushing on surface roughness, fluoride release, and biofilm formation with different tooth-colored materials

doi: 10.1016/j.jds.2021.08.013

Figure Lengend Snippet: Representative images of S. mutans biovolume on different materials from CLSM using FV10-ASW software. A is a Z-projection in the X-Z direction, and B is a Z-projection in the Y-Z direction. The optically combined Z-stack slices are shown in C. The yellow lines in C indicate orthogonal planes of the X-Z and Y-Z projections, respectively.

Article Snippet: The cariogenic bacteria S. mutans (ATCC 25175, Thai Can Biotech, Bangkok, Thailand) were cultured in brain-heart infusion agar (BHI) in a 5% CO 2 incubator at 37 °C for 48 h. Bacteria were then cultivated in brain-heart infusion broth supplemented with 5% sucrose in 5% CO 2 at 37 °C to achieve the desired turbidity at a cell density of 1 × 10 8 CFU/ml or 0.5 McFarland.

Techniques: Software

Journal: Journal of Dental Sciences

Article Title: Effect of brushing on surface roughness, fluoride release, and biofilm formation with different tooth-colored materials

doi: 10.1016/j.jds.2021.08.013

Figure Lengend Snippet: S. mutans live/dead ratios (means ± standard deviation) in biofilms on the tested materials.

Article Snippet: The cariogenic bacteria S. mutans (ATCC 25175, Thai Can Biotech, Bangkok, Thailand) were cultured in brain-heart infusion agar (BHI) in a 5% CO 2 incubator at 37 °C for 48 h. Bacteria were then cultivated in brain-heart infusion broth supplemented with 5% sucrose in 5% CO 2 at 37 °C to achieve the desired turbidity at a cell density of 1 × 10 8 CFU/ml or 0.5 McFarland.

Techniques: Standard Deviation

Journal: Journal of Dental Sciences

Article Title: Effect of brushing on surface roughness, fluoride release, and biofilm formation with different tooth-colored materials

doi: 10.1016/j.jds.2021.08.013

Figure Lengend Snippet: Representative images of S. mutans biovolume and live/dead cells under CLSM. The optical slices combining all Z-stack slices are shown. Live and dead cells are shown in green and red, respectively. The combination between live and dead cells is represented in yellow.

Article Snippet: The cariogenic bacteria S. mutans (ATCC 25175, Thai Can Biotech, Bangkok, Thailand) were cultured in brain-heart infusion agar (BHI) in a 5% CO 2 incubator at 37 °C for 48 h. Bacteria were then cultivated in brain-heart infusion broth supplemented with 5% sucrose in 5% CO 2 at 37 °C to achieve the desired turbidity at a cell density of 1 × 10 8 CFU/ml or 0.5 McFarland.

Techniques:

Journal: Plants

Article Title: Sweet Basil ( Ocimum basilicum L.)―A Review of Its Botany, Phytochemistry, Pharmacological Activities, and Biotechnological Development

doi: 10.3390/plants12244148

Figure Lengend Snippet: Minimum inhibitory concentration (MIC) value of antibacterial activity of O. basilicum L.

Article Snippet: Staphylococcus mutans (ATCC 25175) , Essential oil , 11 mm , [ ] .

Techniques: Concentration Assay, Activity Assay

Journal: Plants

Article Title: Sweet Basil ( Ocimum basilicum L.)―A Review of Its Botany, Phytochemistry, Pharmacological Activities, and Biotechnological Development

doi: 10.3390/plants12244148

Figure Lengend Snippet: Diameter of zone inhibition of antibacterial activity of O. basilicum L.

Article Snippet: Staphylococcus mutans (ATCC 25175) , Essential oil , 11 mm , [ ] .

Techniques: Inhibition, Activity Assay